甲胎蛋白（AFP）酶联免疫吸附试验试剂盒
| 产品描述： | AFP 是一种在胚胎期由卵黄囊和肝脏合成的糖蛋白，出生后表达显著下调，成人血清中维持低水平。作为原发性肝癌（HCC）诊断的经典肿瘤标志物，其异常升高与肝癌发生、发展密切相关，同时在生殖细胞肿瘤、肝硬化等疾病中也存在特征性表达，是肿瘤早期筛查与病程监测的核心指标。本试剂盒采用双抗夹心法 ELISA 技术，通过预包被的抗 AFP 单克隆抗体特异性捕获样本中目标蛋白，与 HRP 标记的检测抗体形成夹心复合物后，经 TMB 底物显色实现定量检测。检测范围覆盖 200-10000pg/mL，灵敏度达 100pg/ml，可精准捕捉早期病变中的微量 AFP 波动，且交叉反应率＜3%，有效规避同源蛋白干扰。适用于人血清、血浆、肝癌细胞培养上清等样本，该试剂盒可用于多领域研究：在肝癌早期诊断中，监测高危人群（如肝硬化患者）血清 AFP 动态变化，辅助识别癌前病变；在抗肿瘤药物疗效评估时，追踪化疗 / 靶向治疗期间 AFP 水平，关联肿瘤体积变化验证药效；胚胎发育机制研究中，通过分析孕期羊水 AFP 浓度探索与胎儿神经管畸形的关联；还能用于肿瘤发生机制解析，探索 AFP 在肝癌细胞增殖、转移中的调控作用及信号通路。为肿瘤学基础研究与相关科研转化提供可靠定量工具 |
| 别名： | 甲胎蛋白、α- 胎儿蛋白 |
| 缩写： | AFP |
| Uniprot No： | P02771 |
| 种属： | Mus musculus (Mouse) |
| 样本类型： | serum, plasma, tissue homogenates, cell culture supernates |
| 检测范围： | 0.5-256pg/mL |
| 反应时间： | 1-5h |
| 样本体积： | 50-100ul |
| 检测波长： | 450 nm |
| 测定原理： | quantitative |
| 测定方法： | Sandwich |
| 货期： | 3-5 working days |
| 本试剂盒所含 材料： | A micro ELISA plat 8×12 |
| Two vials lyophilized standarde | |
| Concentrated Enzyme-Labeled Detection Antibody (100 x concentrate) 1 x 120 μl | |
| Universal Diluent 2 x 20 ml | |
| Wash Buffer (20 x concentrate) 2 x 10 ml | |
| TMB Substrate 1 x 10 ml | |
| Stop Solution 1 x 60 ml | |
| Four plate sealing film (For 96 wells) | |
| An instruction manua | |
| 精密度： | ntra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | |
| Three samples of known concentration were tested in twenty assays to assess. |
| 线性度： | To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 97 | |
| Range % | 92-104 | ||
| 1:400 | Average % | 91 | |
| Range % | 82-98 | ||
| 1:800 | Average % | 103 | |
| Range % | 99-111 | ||
| 1:1600 | Average % | 87 | |
| Range % | 82-96 | ||
| 回收率： | The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-98 | |
| EDTA plasma (n=4) | 112 | 105-117 | |
| 标准曲线： | 这些标准曲线仅用于演示。应为每组分析的样品生成标准曲线。 | ||
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| 靶点详情： | AFP 是分子量约 70kDa 的糖蛋白，属白蛋白家族，含三个结构域，可结合脂肪酸、类固醇等小分子。胚胎期由卵黄囊、胎肝合成，胎儿血中浓度达 3-4mg/mL，出生后 1 年降至成人水平（＜20ng/mL）。 |
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