致死性(3)恶性脑瘤样蛋白3（L3MBTL3）酶联免疫吸附试验试剂盒
| 产品描述： | 本试剂盒采用双抗夹心法 ELISA 技术，专为科研场景设计，可定量检测样本中 L3MBTL3 蛋白浓度，检测范围为 100-1000pg/mL。试剂盒包含预包被 L3MBTL3 特异性捕获抗体的微孔板、HRP 标记检测抗体、标准品及配套试剂，具有高特异性（与 MBT 家族其他成员交叉反应率＜1%）和高重复性（批间变异系数＜8%），适用于细胞裂解液、组织匀浆、细胞核提取物等多种科研样本类型。 |
| 别名： | 致死性(3)恶性脑瘤样蛋白3 含 3 个 MBT 结构域蛋白 3、malignant brain tumor domain-containing protein 3 |
| 缩写： | L3MBTL3 |
| Uniprot No： | Q8N122 |
| 种属： | Mus musculus (Mouse) |
| 样本类型： | serum, plasma, tissue homogenates, cell culture supernates |
| 检测范围： | 100-1000pg/mL |
| 反应时间： | 1-5h |
| 样本体积： | 50-100ul |
| 检测波长： | 450 nm |
| 测定原理： | quantitative |
| 测定方法： | Sandwich |
| 货期： | 3-5 working days |
| 本试剂盒所含 材料： | A micro ELISA plat 8×12 |
| Two vials lyophilized standarde | |
| Concentrated Enzyme-Labeled Detection Antibody (100 x concentrate) 1 x 120 μl | |
| Universal Diluent 2 x 20 ml | |
| Wash Buffer (20 x concentrate) 2 x 10 ml | |
| TMB Substrate 1 x 10 ml | |
| Stop Solution 1 x 60 ml | |
| Four plate sealing film (For 96 wells) | |
| An instruction manua | |
| 精密度： | ntra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | |
| Three samples of known concentration were tested in twenty assays to assess. |
| 线性度： | To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 92 | |
| Range % | 90-103 | ||
| 1:400 | Average % | 93 | |
| Range % | 82-101 | ||
| 1:800 | Average % | 98 | |
| Range % | 99-103 | ||
| 1:1600 | Average % | 90 | |
| Range % | 90-96 | ||
| 回收率： | The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 85-98 | |
| EDTA plasma (n=4) | 112 | 99-108 | |
| 标准曲线： | 这些标准曲线仅用于演示。应为每组分析的样品生成标准曲线。 | ||
| 靶点详情： | L3MBTL3 属于 MBT 结构域超家族，为核内蛋白，分子量约 55kDa。其结构特点表现为：N 端串联 3 个 MBT 结构域，负责识别并结合甲基化组蛋白；C 端 SAM（ sterile alpha motif）结构域通过同型或异型相互作用参与蛋白复合体组装，整体结构通过介导染色质高级结构形成，实现对靶基因转录的精准调控，是表观遗传研究领域的重要分子标志物。 |
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