抗转录因子Snail 酶联免疫吸附试验试剂盒
| 产品描述： | Snail 是一类高度保守的锌指转录抑制因子，在胚胎发育的上皮 - 间质转化（EMT）过程中起核心调控作用，其异常激活与肿瘤细胞侵袭转移、器官纤维化等病理进程密切相关，是解析细胞表型转换分子机制的关键靶点，在肿瘤生物学与发育生物学研究中具有重要科研价值。本试剂盒采用双抗夹心法 ELISA 技术，通过预包被的抗 Snail 单克隆抗体特异性捕获样本中目标蛋白，与辣根过氧化物酶标记的检测抗体形成夹心复合物后，经 TMB 底物显色实现定量检测。检测范围覆盖 15.62-1000pg/mL，可精准捕捉不同实验模型中 Snail 的表达波动，满足低丰度样本的检测需求。在研究机制层面，Snail 通过结合 E - 钙粘蛋白等上皮标志物基因的启动子区域，抑制其转录表达，同时上调波形蛋白、N - 钙粘蛋白等间质标志物，推动细胞从极性上皮表型向具有迁移能力的间质表型转换。本试剂盒通过特异性识别 Snail 蛋白构象表位，可准确反映其在细胞转分化过程中的表达水平变化，为 EMT 相关信号通路研究提供量化依据。 |
| 别名： | Snail 家族锌指蛋白 1、Snail1、锌指蛋白 SNAI1 |
| 缩写： | SNAIL |
| Uniprot No： | O95835 |
| 种属： | Mus musculus (Mouse) |
| 样本类型： | serum, plasma, tissue homogenates, cell culture supernates |
| 检测范围： | 0.5-256pg/mL |
| 反应时间： | 1-5h |
| 样本体积： | 50-100ul |
| 检测波长： | 450 nm |
| 测定原理： | quantitative |
| 测定方法： | Sandwich |
| 货期： | 3-5 working days |
| 本试剂盒所含 材料： | A micro ELISA plat 8×12 |
| Two vials lyophilized standarde | |
| Concentrated Enzyme-Labeled Detection Antibody (100 x concentrate) 1 x 120 μl | |
| Universal Diluent 2 x 20 ml | |
| Wash Buffer (20 x concentrate) 2 x 10 ml | |
| TMB Substrate 1 x 10 ml | |
| Stop Solution 1 x 60 ml | |
| Four plate sealing film (For 96 wells) | |
| An instruction manua | |
| 精密度： | ntra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | |
| Three samples of known concentration were tested in twenty assays to assess. |
| 线性度： | To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 99 | |
| Range % | 95-101 | ||
| 1:400 | Average % | 96 | |
| Range % | 82-100 | ||
| 1:800 | Average % | 99 | |
| Range % | 95-111 | ||
| 1:1600 | Average % | 89 | |
| Range % | 86-98 | ||
| 回收率： | The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 85-101 | |
| EDTA plasma (n=4) | 112 | 100-108 | |
| 标准曲线： | 这些标准曲线仅用于演示。应为每组分析的样品生成标准曲线。 | ||
| 靶点详情： | Snail 是锌指转录因子家族的重要成员，分子量约为 29kDa。该蛋白的核心功能结构域分工明确：锌指结构域通过特异性识别 DNA 序列，介导其与靶基因启动子结合；N 端结构域则参与转录抑制复合体的组装，二者协同作用调控基因表达。在生物学进程中，Snail 可通过调节上皮 - 间质表型转换（EMT）相关基因的表达，驱动细胞发生形态与功能的转变，在胚胎发育、肿瘤转移、组织修复等过程中，对细胞迁移和分化起着关键调控作用。因此，Snail 作为细胞生物学研究的重要分子标志物，在揭示细胞行为机制、疾病发生发展机制等领域具有重要研究价值。 |
上一篇：SNCA-ELISA试剂盒
下一篇：L3MBTL3-ELISA试剂盒
鲁公网安备37132402371612号